FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35684026
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT47750.010.1147311
CCCGTAT34300.09.5914071
TCGGGTC59550.09.4037582
CGCGTAA11950.09.3719651
CGTATCG28800.08.9932453
CTCGGAT79600.08.925481
TCGCTCG46400.08.900475522
CTCGGGT79500.08.8046371
CGCCAAT68900.08.5337251
CTCGAAT62800.08.4709581
CTCGTTT58450.08.4427361
CGACCTA20850.08.3934274
CTCGTAT32450.08.3047121
TTACACG36750.08.18979932
CTCGACT40700.08.1691671
CGCGCTA21700.08.06413246
CTTGGAT217700.07.97391941
TAAGTCG35250.07.942806220
CGAAGAT113650.07.9142961
GTATCGA31550.07.87651974
Produced by FastQC (version 0.11.5)