FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28991685
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG42600.014.49561645
CGTATCT49900.012.58228739
TATCTCG48400.012.40215441
TATGCCG50500.012.36450248
TCCGTAT49950.011.94809337
GTTCCGT53300.011.84455135
CTCGTAT53900.011.84066744
CGTATGC53850.011.53130646
CCGGGAT75800.010.7209191
ACACGTC62900.010.47545513
CCGTATC57950.010.35818838
TTCCGTA63000.09.91119536
TCGCTCG45350.09.73698314
CGCGATT9000.09.6057411
CCGGAAT47200.09.59761
GATCGGA62100.09.5222111
CGAAGAT88000.09.3524971
TCTCGTA70500.09.19945143
CGCATAT12800.09.1854891
CGCGAAC11800.09.0847511
Produced by FastQC (version 0.11.5)