FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35132325
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG32000.015.1921923
CCCGTAT40500.013.4635981
GTATCGA38150.012.8292254
CGCGTAA19150.012.1786081
GCGGTAT54600.011.9118891
AATCGGG51100.011.5709165
CAATCGG44500.011.3676174
TCGATTG37150.011.14030120
TTACGCG15050.010.68648154
CGCGGAT30200.010.5504961
CGGGGTT94350.010.548941
CCGTATC57700.010.5318342
ATCGAAG67250.010.5017646
TATCGAA45350.010.4304095
CGGAAAT61450.010.370221
TCGGGTC66450.010.1336752
GCGTCGT29900.010.10273712
CGTATCA28700.010.06835450
CGCGCGT30750.09.9344981
TACTCGA42300.09.85663917
Produced by FastQC (version 0.11.5)