FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21437857
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG70900.031.05148533
TATGCCG76550.029.53965649
TCGTATG76700.029.52635446
CGTATGC77200.029.29094347
AGATCGG74500.029.1773741
GATCGGA79450.027.6046682
ACACGTC87700.027.53569814
CGCACAT75650.027.52488738
AGTCACG89100.026.98926529
GTCCGCA81550.026.57194935
CTCGTAT85650.026.44081145
CGTCCGC84700.026.03769134
TCACGTC94800.025.4027731
GTCACGT94250.025.29761330
ACGTCTG98000.024.85046616
CACGTCT99550.024.25796715
TCTCGTA91500.024.15393844
ATCGGAA91200.024.0850413
ATGCCGT95800.023.92433550
CCGCACA95050.023.20116237
Produced by FastQC (version 0.11.5)