FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21437857
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA49150.044.4291250
CGCCGTA51550.042.49032247
GCCGTAT53650.040.95754648
GCGTCGT56500.040.83767312
GATCGGA65150.037.6063462
CCGTATC60850.036.16992649
CGTGTAG68050.034.417216
TCGTGTA68350.033.71101415
TGGTCGC62500.033.49755543
AGCGTCG70300.033.4617111
ATCGGAA73800.033.1058923
GTCGCCG69100.032.8444145
TCGCCGT66550.032.13471246
AGAGCGT73400.032.004789
GGTCGCC71400.031.93124244
GTAGATC72900.031.55795532
GTCGTGT73200.031.47617714
CGTCGTG76300.030.78557613
AGATCGG79600.029.9130951
TCTCGGT75150.029.23770537
Produced by FastQC (version 0.11.5)