FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32857069
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4431571.3487417273890132TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3896171.185793535022859TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG454240.138247267277553TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAA374440.11396025616283668TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG941000.057.199371
CGTTTCG1700850.034.1398733
TCGTATG1697150.033.97963345
TATGCCG1696000.033.97012748
CGTATGC1702600.033.87086546
ACACGTC1729750.033.8682213
CTCGTAT1698350.033.7778144
ACGTCTG1734450.033.74263815
GTTTCGG1726750.033.61980434
AGTCACG1719600.033.6107128
CGGAATC1700300.033.56467438
GTCACGT1717950.033.5164229
GCCGTCT1694850.033.49036851
AATCTCG1710000.033.4726641
CACGTCT1747500.033.46893714
TTCGGAA1741600.033.43229336
TTTCGGA1736600.033.3995235
ACGTTTC1737700.033.3956332
TGCCGTC1705850.033.3937450
TCGGAAT1726100.033.3827337