FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32857069
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA597170.18174779984179357Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG804750.059.1610031
CGGCGTG192700.058.27655412
CGAAACG171600.057.201527
CGGCATA194900.056.62744512
CATACGA175550.056.5019715
ACGGCGT88000.055.27767611
GCATACG199150.054.55278414
GCGGCGT147150.052.93915611
AGACGGC294950.052.5577859
AGCGGCG145050.052.16039310
ATACGAG178800.051.5579316
CCGAAAC231550.049.72040626
GGCGCCG221950.049.7197344
TCGGGGG477000.049.71660238
AAGACGG306700.049.4645848
ACGGCAT243000.049.27897311
GGGGCGC178350.048.6716442
GCGTGCG75950.048.05985614
TCCGAAA252000.047.90343525
TGGTCGC995600.047.46848743
Produced by FastQC (version 0.11.5)