FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28900349
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG42750.018.64170645
TATGCCG45200.017.47803748
CGTATGC49250.016.53162646
CTCGTAT49950.015.88554744
ACGTCCG51250.015.41432232
AGTCACG61350.013.66599828
ACACGTC67400.012.43779613
TCTCGTA65850.012.36420243
GATCGGA61550.011.7269741
GTCACGT72650.011.53984629
CGCACAT67100.011.46482437
ATCTCGT72400.011.34091942
CGTCCGC74500.010.74278233
GTCCGCA73200.010.650804534
ACGTCTG79550.010.62476315
TCGCTCG33650.010.55964314
ATCGGAA68850.010.5228162
TCACGTC75650.010.35157630
CCGGGAT65300.010.2602091
CGAAGAT88400.09.7278031
Produced by FastQC (version 0.11.5)