FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28900349
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT10150.012.5903031
TCGCTCG36850.010.67422414
CGCGGAT28350.010.4772161
CGAAGAT84300.010.0787821
CGGACAT52250.010.0474961
CTCGGAT68950.09.968261
CCGGGAT66850.09.4030891
CGCATAT13600.09.39644051
CGCCAAT53400.09.1843331
CTCGAAT46350.09.0909791
CGTATCA29100.09.00975250
TCGGATC39100.08.6479022
TAAGCGA16400.08.62442246
CGAGAAT52550.08.609921
CGAAAAC36750.08.5523321
CCGAGAT89850.08.4952211
CGGGGAT66600.08.4530591
CCGGAAT47800.08.3816711
CGGGAAT52150.08.3448161
GTCGGTT30200.08.2342881
Produced by FastQC (version 0.11.5)