FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31540813
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA64000.027.70702449
GCCGTAT71800.025.7541447
GATCGGA109700.023.1191231
CGCCGTA79600.023.01379246
CCGTATC83850.021.92960748
GCGTCGT92700.021.32463511
ATCGGAA119050.021.2440382
GTAGATC109300.019.75881631
AGAGCGT114550.018.5219528
GTATCAT99150.018.09328350
TCGTGTA104350.018.08432414
AGCGTCG124100.017.43020410
CATACGA18900.017.15826815
GTCGTGT112900.017.08147213
CGTGTAG123250.017.07459315
TCGGAAG147850.017.0591963
AAGAGCG141500.017.0178897
ATCTCGG152650.016.31725735
GATCTCG149500.016.26876634
TAGATCT133600.016.21660232
Produced by FastQC (version 0.11.5)