FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23146161
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG106000.028.38017545
CTCGTAT112100.026.774344
TATGCCG116900.026.46979148
CGTATCT115250.025.14432739
TATCTCG113850.025.12058441
CGTATGC121200.025.0478546
TCCGTAT120000.024.37902337
GTTCCGT121600.024.31242635
GATCGGA130600.024.2723751
CCGTATC121700.023.8967438
TCTCGTA124550.023.8210643
ACACGTC132650.023.61682513
ATCGGAA136250.023.3389072
TTCCGTA128500.022.9532436
ATCTCGT133900.022.41532542
ACGTCTG142650.022.27528615
CACGTCT144850.021.86588714
AGTTCCG143550.021.79641534
ATGCCGT142150.021.67086449
AGATCGG97850.021.4210431
Produced by FastQC (version 0.11.5)