FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26194108
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44200.037.45679543
CGTATGC48100.034.70718444
TATGCCG48900.034.0687146
GATCGGA54650.031.3176291
ACACGTC57400.030.88046313
TATCTCG54600.030.82759539
CTCGTAT60150.027.63904642
CACGTCT70350.025.44157214
CACTAGC70600.024.76917631
ACTAGCT71100.024.74052232
ATCGGAA71500.023.9270382
TAGCTTA74950.023.46970734
ATGCCGT72800.023.12096247
ACGTCTG78450.022.90620215
TCTCGTA72450.022.56584441
TCGGAAG84900.020.5571823
TCACTAG89400.020.37056530
GCTTATC84150.020.24810836
CACACGT93150.019.43688812
GCCGTCT90100.018.7583849
Produced by FastQC (version 0.11.5)