FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27045305
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG41950.039.0546643
TATGCCG48350.033.9565946
CGTATGC49100.033.6486544
CTCGTAT56250.028.69681542
ACACGTC61950.028.28097513
GATCGGA62050.026.4733721
CACGTCT76850.023.02256814
ATGCCGT77400.021.83578147
ACGTCTG81300.021.67907315
TCTCGTA74950.020.9387241
ATCTCGT78500.019.64037940
CACACGT94450.018.76908512
TCGGAAG91400.018.6047213
ATCGGAA90250.018.2682722
GCCGTCT91300.017.98285349
GACCAAT97300.017.12167735
ACCAATC93800.016.98834836
CGTCTGA105400.016.85302416
GTATGCC102150.016.61270545
AATCTCG96200.016.5286739
Produced by FastQC (version 0.11.5)