FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27045305
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA15700.023.7239715
CGGCATA20100.020.58962812
GCATACG22450.018.74160614
TTACGCG17850.013.33137654
GCGGTAT45150.012.5719941
GTATCGA25300.012.404644
CGTATCG25100.012.3660823
ATACGAG27150.012.1945216
TACGCGA20000.012.07069155
CCCGTAT29300.011.8127361
ATCGAAG55150.010.8184216
GTCGCTA20950.010.3709827
ACGCGAC25100.09.89288356
CGACTAA25150.09.87319728
AATAACG37000.09.6938251
CCGACAT48400.09.5824711
CCCGATA54900.09.57003769
GTTACGC26450.09.38793653
AGACGGC63800.09.2435499
GTGCGAA18100.09.1458930
Produced by FastQC (version 0.11.5)