FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32507828
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40450.017.73988243
CGTATGC42650.017.39103144
TATGCCG43750.017.032646
CTCGTAT51600.014.17398942
TATCTCG54900.013.44741139
GATCGGA53050.013.3324651
CCCGTAT32500.012.9514521
ACACGTC61350.012.82434213
ACCGATG62900.012.39453732
TTACGCG24950.012.16817354
CGCGAAT9650.011.798551
CGTATCG33900.011.7023743
ATCGAAG70600.011.6784666
GCGGTAT47600.011.6697331
TACGCGA25800.011.63345755
GTATCGA33800.011.4308854
ATCGCGT27950.011.35535167
CGATGTA70500.011.30335634
CGCGTAA36950.010.738051
TCTCGTA76450.010.33389341
Produced by FastQC (version 0.11.5)