FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58056819
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG124400.025.70806743
TATGCCG137600.023.3672346
CGTATGC138550.022.9331244
CTCGTAT144600.021.7111742
ACACGTC167250.019.88566213
GATCGGA157200.019.5372351
CACGTCT195500.017.20561414
AGATCGG134550.016.7476981
ACGTCTG204150.016.40900215
TCTCGTA194150.016.29452141
ATCTCGT206950.015.23668440
ATGCCGT215000.015.13153447
CACACGT224900.015.03369612
TCGGAAG224950.014.0644683
ATCGGAA224300.013.7207012
CGTCTGA248800.013.616714516
GCCGTCT239950.013.52938849
GCACACG271200.012.937811
GTATGCC256000.012.93722245
AATCTCG215000.012.8048439
Produced by FastQC (version 0.11.5)