FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58056819
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA70800.031.64727650
CATACGA39550.024.54094115
CGCCGTA95600.024.0544647
GCGTCGT93250.023.96541412
GCCGTAT96700.023.2805448
CGGCATA52200.020.90961612
GCATACG56100.020.19526114
GATCGGA114850.020.099262
CCGTATC121050.018.82434349
AGAGCGT128400.017.9426829
AGCGTCG133200.017.03705211
AGATCGG139600.016.0136281
CGTCGTG149150.015.9562613
GTAGATC143100.015.84708232
TCGTGTA147950.015.59526815
ATACGAG59150.015.47481616
GTCGTGT150900.015.33642214
GTATCAT152200.015.26507151
TGGTCGC148450.015.23531143
CGTGTAG156400.014.81904316
Produced by FastQC (version 0.11.5)