FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15800127
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA19700.013.8569650
GCCGTAT20900.013.223948
GTCGCCG21250.012.68539845
CGCGAAT6500.012.5985771
TTACACG16350.012.59722732
CGCCGTA23250.012.03435347
TCGCTCG24050.011.92274214
GCGTCGT23850.011.45387512
CGAAGAT53750.011.172691
CGTATCG11700.010.79017453
CGCTCGT27300.010.62837415
GATCGGA32200.010.4906621
TCGCCGT30500.09.73319446
TGGTCGC28200.09.68003343
CCGGGAT31050.09.3407331
CCGACAT27350.09.2320461
CCGTATC29800.09.16009749
CGCAGAC43600.09.1563621
CGGAGAT36650.09.1237981
ATTCGCG3754.2514596E-49.0986266
Produced by FastQC (version 0.11.5)