FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24461834
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29400.015.84146145
TATGCCG31550.015.19885648
TCGCTCG39300.014.65959714
CGTATGC34350.013.85993346
TATCTCG38100.012.31466941
CCGGGAT51550.011.858711
ACTCGTG51400.011.67959422
CTCGTAT41400.011.58305944
CGAAGAT79200.011.3817281
TTACGCG6501.8189894E-1211.14629954
TAAGCGA13800.010.99973946
CCCGATA53150.010.70980469
ACACGTC44300.010.66880713
CGCTCGT55900.010.4913815
CTCGTAC55300.010.48067917
CGCAACC60000.010.3498650
TACTCGT56750.010.21374621
CGCAGAC79950.010.1949811
TTCGCTC61000.09.840528513
TAAGTCG25950.09.83789620
Produced by FastQC (version 0.11.5)