FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27294144
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA122700.036.3057331
TCGTATG127200.035.31086743
ACACGTC141100.034.98584413
ATCGGAA131800.033.2693332
TATGCCG137100.032.91843446
ACGTCTG151950.032.62299715
CGTATGC139600.032.15355344
CTCGTAT138950.031.77793142
CACGTCT160250.030.91253314
CACACGT169000.029.414212
AATCTCG119100.029.05056439
GCACACG174250.029.02285411
TCGGAAG151800.028.9539383
ATCTCGT154750.028.84548240
TCTCGTA152750.028.61340741
CGTCTGA185200.026.87737316
GCCGTCT169850.026.63306249
ATGCCGT169800.026.47741747
CCGTCTT175350.025.8948550
CGGAAGA191000.025.3751374
Produced by FastQC (version 0.11.5)