FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26304702
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26850.014.2695211
CGCGTAA8850.013.6507171
CGTATCG22950.012.9344053
GCGTCGT18850.012.26252912
GCGGAAT31000.011.8025251
CCGGTAT34850.011.4890981
GTATCGA23900.011.4089764
GCGGGTT31300.011.2482921
TCGATTG25500.011.2321920
CGTATCA19700.011.20760950
CGCGGAT18850.011.1698981
CGGAAAT43750.011.1242761
CGGTATA18000.011.1220852
GCGGTAT36750.011.082951
CGGTATT33100.011.0537232
CGCGCGT18500.011.0080641
GTCGCTA15500.010.68840727
CGGAGAT66250.010.6806751
ACGCTTA29550.010.50569544
GACGCTA26300.010.4990914
Produced by FastQC (version 0.11.5)