FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29493688
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG46650.013.92906414
TTACACG36700.012.97759132
CGAAGAT100900.012.5037411
CGCTCGT55750.011.89953415
CCGGGAT49700.011.54530051
CGTATCG22200.011.4119983
CGCAGAC77100.011.1634521
CGTTCGC18150.010.87142416
CGGGGAT56000.010.7719141
ACTCGTG62150.010.50972822
CGCGTAT4902.6621456E-710.5091841
TCGTATG27450.010.28648945
CTCGTAC63450.010.18723617
TAAGTCG26100.010.03648420
CCGGCAT49300.09.9229721
ATCGCGT20350.09.69605367
GTATCGA29500.09.581694
CGCAACC72550.09.23762150
CCCGATA70750.09.23222169
ACTTCGC75200.09.18371511
Produced by FastQC (version 0.11.5)