FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21372841
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG111400.028.50017545
GATCGGA126200.027.0881881
CGTCCCG121950.026.78957235
AGATCGG87550.026.0310651
ACACGTC131350.025.80758713
ACCCGTC129650.025.51502632
CTCGTAT116500.025.38116644
ATCGGAA136900.024.9900652
ACGTCTG138100.024.71880115
TATGCCG132350.024.22046548
CGTATGC134400.023.9270746
TCCCGAT128100.023.82706837
GTCCCGA134500.023.80769336
CACGTCT143200.023.76713814
TCTCGTA120650.023.5763743
ATGCCGT138100.023.45868349
CCCGTCC144550.023.02530933
CCGATCT123750.022.82033539
TCGGAAG150850.022.747363
CACACGT144400.022.65678412
Produced by FastQC (version 0.11.5)