FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21372841
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA29050.042.55558415
GCATACG33350.038.3077214
CGGCATA34950.037.4733712
CGTATCA57500.036.82703450
GCGTCGT73300.030.15746512
CGCCGTA71300.030.08154147
GCCGTAT71650.029.8859948
GTCGCCG74200.028.59718745
ATACGAG42750.028.51666816
AGCGTCG82400.027.20527811
CCGTATC80400.026.67934849
TCGCCGT76350.026.64913246
TGGTCGC78600.025.72774543
GATCGGA91000.025.1202642
GGTCGCC86700.024.7148244
CGTCGTG92150.024.51531813
AGAGCGT91850.023.8783179
TCGTGTA92450.023.76413715
AGATCGG95650.023.6830941
ACGGCAT55300.023.55802211
Produced by FastQC (version 0.11.5)