FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30482303
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT27300.032.12590435
CCGTACG32150.028.10002134
ACCGTAC36500.024.84144833
TACGTAA35250.024.50594337
TCGTATG57200.023.86883246
TATGCCG59000.023.02910449
GTACGTA38300.022.72693336
CGTATGC61450.022.43268447
CGTAATC37250.022.0388839
ACGTAAT40800.020.84855538
GATCGGA67350.020.8073222
AGATCGG69250.020.3373371
ACACGTC74200.019.77325614
CTCGTAT73250.018.41297745
ATCGGAA77850.018.1277373
ACGTCTG83650.017.8937216
CACGTCT91150.016.20439315
TCTCGTA83400.015.85551144
AATCTCG56350.015.56331242
CACCGTA61650.014.97483432
Produced by FastQC (version 0.11.5)