FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30482303
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT44100.033.49571612
CGTATCA40850.031.34710950
GCCGTAT44450.029.85729248
GATCGGA53100.028.2623162
AGCGTCG56150.026.18859511
CGCCGTA52350.025.16051347
AGAGCGT65150.023.082689
ATCGGAA65700.022.8421443
CCGTATC59000.022.32436849
GTCGCCG61300.022.0297445
TGGTCGC59000.021.64644443
TCGCCGT56800.021.48659346
CGTGTAG70000.021.18998116
GTCGTGT72300.020.70016514
TCGTGTA73600.019.97183415
AGATCGG73700.019.8645271
CGTCGTG75550.019.59513913
GTAGATC77250.018.5158232
AAGAGCG84350.017.907748
GGTCGCC75700.017.75066844
Produced by FastQC (version 0.11.5)