FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36620358
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG33750.020.66465843
CGTATGC38550.018.17907344
TATGCCG39750.018.08220746
CGTATCG34700.014.2309363
GTATCGA36350.014.1980344
CCCGTAT37900.013.3450991
ATCGAAG73850.013.1724936
GCGGTAT55350.013.1584841
CGCGTAA28500.013.1325141
TTACGCG22100.013.1118354
CTCGTAT58500.013.01621742
ACACGTC59650.012.65486613
GATCGGA57700.012.2135031
TACGCGA25050.011.56776955
CCGTATC51550.010.9163372
GTCGCTA28600.010.72015627
CGCGATT10250.010.1978251
TATCGAA51950.010.0775135
AATCGGG44100.010.0190875
CACGATC71450.09.90257436
Produced by FastQC (version 0.11.5)