FastQCFastQC Report
Fri 10 May 2024
EGAF00004859369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14246165
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1206980.8472315180962735TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG335880.23576871389598533TruSeq Adapter, Index 4 (100% over 49bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG251690.17667210789710774Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)