FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14246165
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA97750.062.41700415
CGGCATA110450.060.46573312
GCATACG110750.060.29495214
ATACGAG99750.057.118516
CGAGATT91600.051.6019419
AAGACGG146800.051.0682078
ACGAGAT94400.050.71040718
ACGGCAT133100.050.44560211
AGACGGC148650.050.4384889
TACGAGA94100.050.17570517
GGCATAC140550.047.88462413
GACGGCA146700.045.77460510
GAAGACG168450.044.19767
CGTATCA63900.038.61231250
CAAGCAG252900.034.6945571
AGATTGG134200.033.6783921
GACGCGT11550.033.12006845
CGGCGTG48050.032.8089612
AGAAGAC226500.032.7897726
GATTGGA76900.032.5460122
Produced by FastQC (version 0.11.5)