FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23788629
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG87300.035.1315843
TATGCCG90300.033.96441746
CGTATGC91900.033.3730944
CTCGTAT96900.031.4018342
ACACGTC101500.030.59153413
GATCGGA97700.029.3163951
AGATCGG68300.028.0918981
TCTCGTA112800.027.06720741
CACGTCT114650.027.02168714
ACGTCTG119200.026.13494715
ATGCCGT118650.025.96533447
CACACGT121750.025.67343912
ATCTCGT125550.024.29096440
GCCGTCT128800.023.7048249
GTATGCC136050.022.84738245
TCGGAAG127350.022.7574373
CGTCTGA140400.022.18859916
GCACACG142750.022.08995611
TGCCGTC139200.022.03295148
GTAATCT131100.021.8942437
Produced by FastQC (version 0.11.5)