FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23788629
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA46700.041.26281750
GCGTCGT52450.036.74320212
GCCGTAT55900.034.3531648
CGCCGTA57950.033.37826547
GATCGGA63400.031.7772642
AGAGCGT69150.028.4688579
AGATCGG70550.028.014871
CCGTATC70750.027.58004849
GTAGATC71700.026.86883732
CGTCGTG76550.026.2135413
GTCGTGT76600.025.70056214
GTATCAT77450.025.23613551
TGGTCGC75600.025.12956843
TCGTGTA78250.025.02574515
CGTGTAG79550.024.96441716
AGCGTCG80100.024.36192111
TCTCGGT80250.023.97159437
GGTCGCC80700.023.9259844
TCGCCGT81250.023.63666346
GAGTGTA78100.023.3024328
Produced by FastQC (version 0.11.5)