FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32340052
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA37600.016.75532250
GCCGTAT42900.015.01165548
GCGTCGT45800.014.74890912
GTCGCCG43600.013.9678945
CGCCGTA47300.013.61522347
TCGCTCG39250.011.68152914
AGCGTCG57050.011.59509311
CGACCGT14550.011.3058421
CGCGGGT18750.011.21
CTCGGGT69500.011.1798571
TAAGCGA23150.011.03671746
TGGTCGC56900.010.94903443
TCGGGTC55500.010.8468482
CCGTATC59150.010.828402549
GTCGTTA25200.010.55555620
CGAAGAT108650.010.5016111
GATCGGA61300.010.4486142
TCGCCGT60400.010.43046446
CCGGGAT52600.010.1140691
CGCGCGT6654.1654857E-1010.0000011
Produced by FastQC (version 0.11.5)