FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39078265
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG73300.017.47660346
TCGTATG74950.017.27799243
CGTATGC77850.016.76988644
CTCGTAT85100.015.46394942
TATCTCG80900.015.22784539
GATCGGA88100.014.1674081
CGATGTA91150.013.78412834
ACACGTC109050.012.54847513
ACCGATG106050.012.54044132
ACGTCTG107450.012.47476115
CACCGAT108900.012.01942331
ATGCCGT111200.011.99215747
ATCTCGT105200.011.94347840
ATCGGAA105250.011.9374222
CGTATCG33200.011.8064183
CACGTCT118850.011.4843214
CCGATGT111250.011.48240133
TCTCGTA113200.011.09955841
CCCGTAT42050.010.839111
GTCACCG136400.09.724442529
Produced by FastQC (version 0.11.5)