FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32023801
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15500.012.8712671
CGTATCG26400.011.9319293
CGTATCA30800.011.59096150
GCCGTAT32500.011.09232248
CGTTCGC24750.010.7474916
GTATCGA32600.010.3068274
CGCGAAA17950.010.13924243
CCGGGAT57050.09.8161791
CGACCTA18450.09.4851674
TCGGGTC57450.09.3212932
GCGTCGT38750.09.30323812
CGCGTTC29100.09.26118214
CCCGTAT37450.09.2525521
CTCGTTT49600.09.2441661
CTCGGGT77200.09.2035821
CTCGGAT72400.09.1369521
TAAGTCG26850.09.124722520
CGCGTAT5058.316856E-69.0101111
CGCCGTA41450.08.78166347
CCGGCAT49600.08.6796371
Produced by FastQC (version 0.11.5)