FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31362988
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA34500.018.76703849
GCCGTAT42350.016.77621847
GATCGGA59900.016.4184281
GCGTCGT45550.014.59994811
ATCGGAA70350.014.07942
GTCGCCG53100.013.70956644
AGCGTCG58350.013.67657310
AGAGCGT66350.011.7106448
CGCCGTA60150.011.28799546
GTATCGA36850.010.6379244
CGTATCG30050.010.5992043
CGTTCGC24450.010.59346916
CCGTATC65000.010.49963348
CGACCTA16850.010.3859554
CGCGTTC25900.010.00036714
CCCGTAT41850.09.9518451
GTAGATC71300.09.86581731
CGCGAAA15350.09.80391943
TGGTCGC58900.09.74480342
TCGGAAG102900.09.592023
Produced by FastQC (version 0.11.5)