FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36581773
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG42050.012.31509914
CGCGTAT6800.011.8509711
CGACCGT15750.011.1230651
CCGGGAT61150.011.0011931
TATGCCG69450.010.5800013
CGCGGGT24900.010.553511
CGCGAAA16750.010.44623443
TAAGCGA23450.010.29770646
GTATCGA38800.010.2803014
CGCGAAC7850.010.2658091
CGTATGC72150.010.198071
CGTATCG28350.010.12044053
CGAAGAT115250.09.8500651
TCGGGTC62900.09.8460332
CGACCTA26650.09.7155464
CGCGAAT15600.09.6578151
CGCTCGT53700.09.64338715
CTCGGGT81000.09.6029121
CCGGCAT58450.09.5312011
CTCGGTT39900.09.4838761
Produced by FastQC (version 0.11.5)