FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28294658
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA25250.018.71715412
GCATACG26500.017.9641614
CATACGA21400.017.56931915
TTACACG35450.014.79407832
CGCCGTA26500.014.58120847
GCGTCGT28450.014.55055612
CTCGGGT61850.014.2789241
CGTATCA26950.014.20826250
TATCGCG6400.014.01401122
CGAAGAT95650.013.8497351
TCGGGTC49750.013.7996822
GCCGTAT30300.012.7521848
ATACGAG28450.012.60938816
TCGCTCG30350.012.16182714
CTCGGTT35200.012.0547341
TACACGA47600.011.81622833
GTACGAT17850.011.01557725
CTCGTTT45050.010.9505631
ACGAAAG53550.010.69666536
GATCGGA45300.010.6620052
Produced by FastQC (version 0.11.5)