FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20282671
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG536700.060.4369741
CGTATCA541500.056.28397850
TGGTCGC536500.055.90068443
CGCCGTA549850.055.5995747
GTCGCCG553900.055.4866145
GCGTCGT572850.055.33880212
TCGCCGT535700.055.24337846
GCCGTAT555800.055.0287448
TCTCGGT572400.054.47411337
CCGTATC563300.054.3029849
CGGTGGT556450.054.1089540
GGTCGCC569500.054.07676344
GAGTGTA536850.053.95031428
GTGGTCG560600.053.5953842
CGTCGTG591200.053.56838213
TCGTGTA589500.053.07726315
GTCGTGT590000.053.0483314
GTATCAT573900.053.02215251
AGCGTCG603000.052.98585511
TCGGTGG591600.052.8405239
Produced by FastQC (version 0.11.5)