FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34761104
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG54800.022.35478443
TATGCCG57400.021.40311446
CGTATGC62500.019.54467244
CTCGTAT61650.019.3031542
GATCGGA63700.017.28331
ACACGTC73600.016.73761213
CGCGAAA14950.015.68615843
TCTCGTA75000.015.26054841
ACGTCTG82650.014.94722115
ATCGGAA75600.014.4894272
CACGTCT92650.013.63610714
ATCTCGT88800.012.88895840
ATGCCGT103400.012.05063547
CACACGT117550.010.47969512
GCCGTCT120200.010.39544349
CGTTCGC25950.010.38441416
TCGGAAG114700.010.1298573
GCACACG136500.010.07600911
CTCGGTT43650.09.9603341
CGTCTGA125100.09.95912816
Produced by FastQC (version 0.11.5)