FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25527306
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG125600.035.2326343
TATGCCG129100.034.38315646
CGTATGC130100.034.040244
TATCTCG135600.031.3744439
GATCGGA148250.031.1326681
CTCGTAT143900.030.70419542
ACACGTC151350.030.5670313
TCTCGTA145950.029.68810341
CGATGTA148850.029.63595234
ACCGATG154450.029.57752832
ATGCCGT155150.029.00525347
ACGTCTG160800.028.98282215
ATCGGAA160500.028.7817732
ATCTCGT154150.028.13097640
CACGTCT166500.028.01110614
GCCGTCT154250.027.83798449
CACCGAT167150.027.452731
CCGTCTT158550.027.29946550
CCGATGT164750.027.04499833
CACACGT182600.025.50397512
Produced by FastQC (version 0.11.5)