FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25527306
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA21950.032.52645515
CGTATCA63350.032.36486450
GCCGTAT66700.031.20296148
GCGTCGT75600.029.02245512
CGCCGTA72350.028.90911947
GCATACG27350.028.3590714
CGGCATA29200.027.88226112
TGGTCGC76000.027.17264643
CCGTATC80500.025.94040549
GTCGCCG84150.025.5949845
AGCGTCG88350.025.25525711
TCGCCGT80250.024.98998646
AGATCGG93950.024.4655281
GATCGGA97700.023.808652
CGTCGTG100250.022.09273313
GGTCGCC97150.022.0732544
AGAGCGT99550.022.0682759
GTAGATC100750.021.75074232
ATCGGAA106850.021.576353
TCGTGTA102250.021.35213515
Produced by FastQC (version 0.11.5)