FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24281613
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG76300.045.5331143
CGTATGC81300.042.6907444
GTCACGA85850.041.43800429
TATGCCG84900.040.92106246
GATCGGA89700.039.3977131
AGTCACG90500.039.37991728
ACACGTC91500.039.05628213
CTCGTAT89900.037.46562242
CACGTCT104000.034.49354614
CACGATC104750.033.863631
ACGTCTG108450.033.20438815
ATGCCGT107500.032.28624347
TCACGAT109750.032.22714230
TCTCGTA103750.031.70613741
CACACGT113350.031.67842112
ACGATCA113900.031.35344332
ATCGGAA112450.031.2378372
TCGGAAG116200.030.5475393
GCCGTCT116000.029.68461849
ATCTCGT110550.029.6322140
Produced by FastQC (version 0.11.5)