FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24281613
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA24050.028.81721115
CGGCATA28850.027.38503312
GCATACG27300.026.27259414
ATACGAG34450.018.71717616
TATCGCG18900.018.26116622
CGTATCA20500.016.162550
AGACGGC66000.015.4747719
CCCGTCG50750.014.8142631
ACGGCAT56000.014.47782811
CGCGTAA19100.014.0838331
CGAGCGA50000.013.93544156
CCGTCGG53350.013.5763222
CCCGTAT24000.012.7890531
GACGGCA72350.012.63826210
CGTATCG22250.012.0909893
ATTCCGT46700.012.04381853
CGTCGGC63000.011.9347473
CGACCAA62050.011.50523160
GTCGCTA18650.011.47335927
AAGACGG86150.011.41548
Produced by FastQC (version 0.11.5)