FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15472817
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA403330.2606700512259662TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA285960.18481443941332726TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG95800.040.8827441
TCGTATG168850.037.22743245
CGTATGC170650.036.90030346
TATGCCG170600.036.80999448
ACACGTC175300.035.68263613
CTCGTAT174900.035.62362744
GATCGGA182500.035.2878841
ACGTCTG178550.035.0100815
TATCTCG178650.034.6828641
CACGTCT181550.034.4162314
ATGCCGT183700.034.1098749
ATCTCGT182850.033.65979842
CACACGT188700.033.55098312
GCCGTCT186050.033.53066351
ATCGGAA192400.033.4424442
TGCCGTC189400.032.99224550
GCACACG195900.032.81100511
TCTCGTA189150.032.61133243
CCGTCTT191800.032.56152
TCGGAAG200900.032.09623
Produced by FastQC (version 0.11.5)