FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15472817
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG94900.040.9554861
GCGTCGT109950.034.78610612
CGTATCA108300.034.5549950
GCCGTAT109900.033.98673248
GTCGCCG109450.033.8781345
CGCCGTA111200.033.68565447
TGGTCGC108750.033.5974843
CATACGA21400.033.31622315
AGCGTCG118450.032.49476611
GCATACG25050.031.91909414
GGCGCCG29150.031.896744
TCTCGGT121950.031.78199437
CCGTATC119300.031.6278749
CGTCGTG122450.031.37664613
TCGGTGG126100.030.90897639
TCGCCGT115800.030.89070946
AGAGCGT125350.030.841579
CGGCATA25850.030.45024712
CGGTGGT121000.030.38907240
TCGTGTA125550.030.12206815
Produced by FastQC (version 0.11.5)