FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27209681
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA61800.041.9745315
CGGCATA65650.039.93338812
GCATACG67800.038.463214
ATACGAG73950.034.6114516
ACGGCAT100300.027.68513711
AAGACGG120100.026.5678738
TACGAGA85850.026.2789217
AGACGGC123950.026.1878839
ACGAGAT87950.025.25904318
GGCATAC120300.022.36581413
GACGGCA132750.022.00909610
CGTATCA30450.021.86919250
GCCGTAT49850.020.97159468
CGAGATC107300.020.7677819
GAAGACG159150.020.4172277
TCGTGAC49350.020.20277829
CGCCGTA58450.018.12187267
CGTGACT57400.017.97048230
TGATCGT60100.017.16186126
TGGTCGC62950.017.15316463
Produced by FastQC (version 0.11.5)