FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35431167
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA24100.016.7633041
CGTATCG32050.014.1577173
CCCGTAT35000.012.8893841
TCGTATG21250.012.49492343
TTACGCG25550.012.36537554
GTATCGA37200.011.8954454
CGGTATT42900.011.75519852
CGACCTA15800.011.6898764
TACGCGA28150.011.4621755
GCGGTAT66100.011.4088531
TCGGGTC77250.011.3589462
GCGTAAC38050.011.0446352
CCGTATC51300.010.61682
GTTCGTT68500.010.56453130
CCGGTAT47900.010.4723981
CCGGGAT75300.010.4620371
CGCGTAT6851.2732926E-1110.3210551
TCGTTCG39550.010.21901228
GCCGTAT27550.010.1426611
CGGTATA21900.010.1320152
Produced by FastQC (version 0.11.5)