FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35431167
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG36000.015.6288643
CGCGTAA27200.014.97460651
CCCGTAT40100.013.1701091
GTATCGA36400.012.7064694
GCGGTAT62250.012.365391
CCGTATC57950.011.7342432
CGACCTA17850.011.4086754
TTACGCG24400.011.02169254
ATCGAAG71900.010.8969496
GACGCTA45400.010.8718044
TCGGGTC75050.010.8083522
TCGTTCG40100.010.67284828
GCCGTAT32950.010.5805511
GTTCGTT75800.010.56130230
CGCGCGT37250.010.3784881
TTACACG40000.010.3494132
GCGTAAC41950.010.1208152
GCGATCG46900.010.07548734
CGATCCA50100.09.9896834
CGGAAAT65550.09.9524831
Produced by FastQC (version 0.11.5)