FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20252254
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA328770.16233748599044828TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG175300.053.2339945
CGTATGC177000.051.9820546
TATGCCG174750.051.92090248
CTCGTAT181250.051.02963644
ACACGTC206000.051.0116713
GATCGGA176850.049.9120031
CACGTCT217550.048.35085714
TCTCGTA193400.047.8239143
ACGTCTG223800.047.04658515
CACACGT228000.046.22567412
ATGCCGT193400.045.48700749
ATCTCGT209050.044.7717842
TCGGAAG204200.043.6772883
GCCGTCT201100.043.38508251
GCACACG244600.043.3000911
ATCGGAA210450.042.035862
GTATGCC221700.041.70352647
CGTCTGA253900.041.6186516
CGGAAGA253400.041.4584734
AATCTCG225700.041.407941
Produced by FastQC (version 0.11.5)