FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20252254
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA37600.039.708850
CATACGA17250.037.6265915
CGGCATA23200.035.41540512
GCGTCGT46450.032.99897812
GCATACG24200.032.13350314
CGCCGTA50650.030.23271847
GCCGTAT51050.029.79166648
GATCGGA54850.029.2231142
AGAGCGT59450.026.2027599
CCGTATC59350.025.56653849
GTATCAT61900.025.23346551
AGATCGG62500.024.6609521
GTAGATC61350.024.59127232
ATACGAG23200.024.25691216
TCTCGGT67800.022.23228537
AGCGTCG70900.021.86333311
CGTCGTG72650.021.76426313
TGGTCGC69750.021.58740843
TCGGAAG76400.021.2643224
AAGAGCG75800.021.238938
Produced by FastQC (version 0.11.5)